Key points for the iN Protocol:

• Forced expression of Ngn2 (lentivirus)
• Rapid induction
• Specifically layer 2/3 excitatory neurons, no astrocytes
• Lack of developmental critical windows (as in EB protocol)
• Easily scalable

	matrigel_protocol_v1.pdf	235 KB
	ngn2_protocol_v7_3_2019.pdf	285 KB