Key points for the Embryoid Body Protocol:

• Default ectoderm pathway
• Gives rise to a mix of different forebrain neuronal subtypes (excitatory and inhibitory)
• Gives rise to endogenous astrocytes
• Spontaneous synaptic activity (late-stage)

Critical Windows for the Embyoid Body Protocol:

• Neural Progenitor Cells: ~Days 15-18
• Immature Neurons: ~Day 40+
• “Mature” Neurons: ~Days 80-100+

embryoid_body_protocol_040618.pdf

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Patterning to Neuronal Subtypes

Information coming soon!