April 24, 2020

Dang et al. showed the potential for using cerebral organoids combined with single-cell RNA sequencing to study the effects of drug-induced inflammation.

• analyzed 20,758 cells from eight untreated and six METH-treated cerebral organoids and found that the organoids developed from embryonic stem cells contained a diverse array of glial and neuronal cell types
• identified transcriptionally distinct populations of astrocytes and oligodendrocytes within cerebral organoids
• elicited novel astrocyte-specific gene expression networks regulating responses to cytokines, and inflammasome